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| Ndungu, K; Gitonga, P; Mulinge, M; Kangethe, J; Kibugu, J. K; Munga, L. K; Maina, N; Kagira, J. M; Ngae, G. N; Murilla, G. |
| The efficacy of Triladyl®, a commercial cryomedium for bull semen, in the cryopreservation of both human and animal infective trypanosomes as compared to EDTA Saline Glucose (ESG) 10% glycerol was evaluated in the current study. Cryopreserved Trypanosoma brucei rhodesiense, T. evansi, T. b. brucei and T. congolense were first propagated in irradiated mice. At the peak of parasitemia, parasites were harvested by cardiac puncture and 106,105,104103,102 and 10 dilutions made using whole blood bled from clean mice. These dilutions were divided into two equal portions of 0.5 ml each and cryopreserved in both ESG 10% glycerol and neat Triladly®. The procedure was also repeated with T. congolense and T. vivax species of trypanosomes directly isolated from... |
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Palavras-chave: Trypanosoma; Cryopreservation. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2500 |
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